AB-3000B Cellgraph is an imaging system to detect low-level light emission in a single living cell by using a highly sensitive EM-CCD camera.
Purpose and Application
- Real time reporter gene assay
- Transcriptional factor activity, Clock gene (chronopharmacology). Gene expression (Transfection,RNAi,etc)
- Analysis of cell responses
- Drug response(Anti-cancer drug,DDS,etc),Stress response (Hormone,Inflammation,Anti-Oxidant), Cytotoxichology
- Analysis of signal pathway
- Signal cascade (Calcium), Apoptosis,etc
- Acquire images of culturing cells or tissue samples on a 35 mm dish in the living state.
- Provide a CO 2 incubator equivalent atmosphere by controlling the temperature, CO 2 gas concentration and humidifying.
- Highly sensitive and accurate detection of faint bioluminescence with an EM-CCD camera.
- Isolates and captures multicolor faint bioluminescence.
- Easy operation via a user-friendly interface.
- Full control from a PC with dedicated software.
- Completely lightproof,compact design.
|Cooled CCD Camera||CCD type: Back-illuminated EMCCD|
|Active pixel: 512 x 512 pixel|
|Pixel size: 16 x 16μm|
|AD Resolution: 14/16 bit|
|-80°C with an air-cooling system when the air temperature is 25°C, -90°C with a water-cooling system*|
|(when the water temperature is 25°C)|
|-85°C with an air-cooling system when the air temperature is 20°C, -100°C with a water-cooling system*|
|(when the water temperature is 16°C)|
|*The water-cooling unit is provided as an optional accessory.|
|Objective Lens||4x (NA 0.53) (Other magnification lenses are also available as optional accessories)|
|Stage||X-Y-Z axis manual stage, Z-axis motorized stage|
|Culture dish||35mm culture dish|
|Constant temperature function||(Room temperature +5°C) to 45°C, 0.1°C step|
|Lighting||White LED with dimming function Blue LED with dimming function|
|Optical Filter||Up to three filters can be set (525LP/560 LP/620 LP are standard equipment)|
|Exposure time||30 milliseconds to 90 minutes|
|Control software||OS：Windows 7/Vista/XP|
|System requirement||RAM: 1 GB or more (512 MB or more for XP)|
|Hard Drive: 30 GB or more free disk space required|
|Interface: Full size PCI slot x1, serial port x1, USB port x1|
|Power (main body)||AC100-240V 106VA (varies according to the components of the whole system)|
|Dimensions (main body)||430 mm (W) x 600 mm (D) x 650 mm (H)|
|Weight (main body)||Approx. 40kg|
|CCD camera water-cooling unit||Constant-temperature water circulating system, heat-resistant tube for circulation|
|CO 2 gas injection unit||CO 2 gas-mixer|
|CO 2 gas humidifying unit||Bubbling system for gas humidification|
|Perfusion culture chamber unit||Chamber for perfusion|
|Objective lenses||2x, 10x, 20x, 40x, 60x|