AE-1390 SDS-eliminant

AE-1390 SDS-eliminant is the reagent for removing SDS (Sodium dodecyl sulfate) from samples including SDS by 2 steps.

AE-1390 SDS-eliminant is the reagent for removing SDS (Sodium dodecyl sulfate) from samples including SDS by 2 steps. At first, Solution A removes dodecyl sulfate ion to precipitate it by insoluble pellet. The SDS also bound to proteins directly, however, could not be removed by Solution A. Second, the non-ionic surfactant including Solution B makes mixed micelle with SDS. This step additionally removes the SDS direct-binding to the proteins. SDS-eliminant is extremely useful method in sample isolation, fractionation and purification.

AE-1390 SDS-eliminant  

AE-1390 SDS-eliminant

Purpose and Application
  • Removal of SDS from the sample solution
  • Removal of SDS from the proteins isolated SDS-PAGE
  • Removal of SDS from the cell or tissue extracts
  • Preparation of Mass Spectrometry, electrophoresis and chromatography
  • Reactivation of enzymes* denatured by SDS (* not all enzymes)
Product Summary

Solution A removes dodecyl sulfate ion to precipitate it by insoluble pellet. The SDS also bound to proteins directly, however, could not be removed by Solution A. Second, the non-ionic surfactant including Solution B makes mixed micelle with SDS. This step additionally removes the SDS direct-binding to the proteins.

Features
  • Ready to Use
  • Over 95% of the ionized SDS is removed briefly
  • The recovery success rate of proteins is high
  • Available for small amount samples (less than 1mL)
Specifications
Product number AE-1390
Product name SDS-eliminant
Components Solution A Solution B
Major constituent KCl、Tris non-ionic surfactant、Tris
Package 2mL solution / a tube 2mL solution / a tube
Max sample volume 40mL 10mL
Storage Cold storage in light-blocking bag, one year guarantee