AE-1430 EzApply

AE-1430 EzApply is a sample preparation buffer for SDS-polyacrylamide gel electrophoresis (SDS-PAGE) of proteins.

AE-1430 EzApplyAE-1430 EzApply

Purpose and Application
  • Extraction of Protein and PolyPeptide
  • Sample buffer for SDS-PAGE
Features
  • Use of dithiothreitol (DTT) as a reducing reagent
  • Reduction reaction more secure than 2-Me
  • Less likely to produce false bands than 2-Me
  • No smell like 2-Me* (*2-Me: 2-mercaptoethanol)
  • Low running cost and simple preparation procedure
Specifications
Product Number AE-1430
Product Name EzApply
Major components Tris-HCl buffer, 2% SDS, 20% sucrose, BPB, and 100 mM DTT
(final concentrations: about halves of the above figures)
Package Solution/30 mL bottle (EzApply solution)
Solution/5 mL bottle x 5 bottles (DTT)
Method of use EzApply solution is added to DTT bottles and mixed with sample at a ratio of 1:1
Applicable amount 5 mL x 5
Storage period At –20ºC for 6 months (before dissolved in DTT) or 1 week after dissolved with DTT

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