c-PAGEL compact gel (60×60 mm)

Compact size (60×60 mm).

c-PAGEL is precast polyacrylamide gels of compact size (60×60 mm) offering easy operation, fast sample run time, and efficient separation, in combination with ATTO compact polyacrylamide gel electrophoresis system.

c-PAGEL compact size precast polyacrylamide gels
c-PAGEL compact size precast polyacrylamide gels
Purpose and Application
  • Separation and detection of proteins (C type)
  • Second step of 2D electrophoresis (CD type)
  • SDS-PAGE, Native-PAGE
  • Detection of PCR products, separation of DNA
  • Screening
  • Experimental training for students or researchers
Features
  • Compact size (60 mm x 60 mm)
  • Fast electrophoresis run time as 30 min
  • Even with small amount of samples and reagents, gives good separation
  • Offers stability and reproducibility
  • Glass plates with good thermal stability and uniformity employed
  • Easy to set on to the apparatus and take off the gel from the glass plate
  • Varieties of gels are available, including wide range of gel percentage, wells, gels for 2D electrophoresis also available
Specifications
Product Number C7.5L/C10L/C12.5L/C15L/C520L/C15S CD7.5L/CD10L/CD12.5L/CD15L/CD520L
Product Name c-PAGEL(R) c-PAGEL(R)
Usage Polyacrylamide Gel Electrophoresis of Protein or Nucleic Acid (SDS-PAGE, native PAGE)
Glass Plate Size 76mm(W)x70mm(H)
Gel Size 60mm(W)x60mm(H), Thickness 0.75mm
Gel/Buffer Composition Polyacrylamide, Tris-HCL Buffer
Gel Percentage and
Separation Range in Molecular Weight
Wells Style Gel Percentage Separation Range of Molecular Weight
c-PAGEL分画分子量範囲
12 Wells For 2D Protein (Da) DNA  (bp)
C7.5L CD7.5L 7.5% 35,000 – 400,000 250 – 3,000
C10L CD10L 10% 25,000 – 300,000 150 – 2,000
C12.5L CD12.5L 12.5% 10,000 – 250,000 70 – 1,800
C15L 15% 5,000 – 200,000 50 – 1,500
C520L CD520L 5 – 20% 5,000 – 400,000 50 – 3,000
C15S 15% 1,000 – 100,000
Sample Wells
Sample Loading Volume
12 Wells (Width 2.9 mm)
Maximum Loading Volume: about 8μL/well
No Well
Gel Width 55 mm (depending on the first step IEF gel length)
Transportation/ Preservation Condition Can be transported in room temperature. Should be stored in a refrigerator (Caution: NOT to be frozen)
Shelf Life 6 months when stored in a refrigerator (NOT to be frozen)
Package 10 plates per box

Note 1: Gels do NOT contain SDS. Electrophoresis with buffer solution containing 24mM Tris + 192mM Glycine + 0.1% SDS is Laemmli method, and one without SDS is Ornstein-Davis method.
Note 2: Gels for 2D is flat on top (no wells), on to which the first step IEF gel is easy to set.
Note 3: Use buffer solution containing 25mM Tris and 192mM Glycine for DNA electrophoresis.
Note 4: Use buffer solution containing 100mM Tris and 50mM Tricine and 0.1% SDS for electrophoresis with E-T15S/E-R15S.

You may also like...