Scientific equipments and tools

Peristaltic pumps, chromatograph equipments and electrophoresis apparatus

PAGEL mini gel (90×78 mm)

by · January 19, 2015

Mini size (90×78 mm).

PAGEL is precast polyacrylamide gels of  mini size (90×78 mm) offering easy operation and efficient separation, in combination with ATTO mini polyacrylamide gel electrophoresis system.

PAGEL mini size precast polyacrylamide gels
PAGEL mini size precast polyacrylamide gels
Purpose and Application
  • Separation and detection of proteins
  • Second step of 2D electrophoresis
  • SDS-PAGE, Native-PAGE
  • Detection of PCR products, separation of DNA
  • Screening
  • Experimental training for students or researchers
Features
  • Mini size (90 mm x 78 mm)
  • Offers stability and reproducibility of separation
  • Glass plates with good thermal stability and uniformity employed
  • Easy to set on to the apparatus and take off the gel from the glass plate
  • Varieties of gels are available, including wide range of gel percentage (from low to high molecular weight) and wells (12, 18, 21 wells)
Specifications
Product Number NPU-5L/NPU-7.5L/NPU-10L/NPU-12.5L/NPU-15L
NPG-310L/NPG-520L/SPG-520L/NPG-1020L		 NPU-R5L/NPU-R7.5L/NPU-R10L/NPU-R12.5L/NPU-R15L
NPG-R310L/NPG-R520L/NPG-R1020L		 NPG-G520L
Product Name PAGEL(R) (12 wells) PAGEL(R) (18 wells) PAGEL(R) (21 wells)
Usage Polyacrylamide Gel Electrophoresis of Protein or Nucleic Acid (SDS-PAGE, native PAGE)
Glass Plate Size 120mm(W)x100mm(H)
Gel Size 90mm(W)x78mm(H), Thickness 1mm
Gel/Buffer Composition Mainly Polyacrylamide, Tris-HCl Buffer
Gel Percentage and
Separation Range in Molecular Weight
Product Number Gel Percentage Separation Range in Molecular Weight
Protein (Da) DNA (bp)
NPU-5L/NPU-R5L 5% 50,000 – 800,000 250 – 5,000
NPU-7.5L/NPU-R7.5L 7.5% 35,000 – 400,000 100 – 2,000
NPU-10L/NPU-R10L 10% 20,000 – 300,000 50 – 800
NPU-12.5L/NPU-R12.5L 12.5% 10,000 – 250,000 30 – 400
NPU-15L/NPU-R15L 15% 7,000 – 200,000 20 – 300
NPG-310L/NPG-R310L 3 – 10% 20,000 – 700,000 200 – 5,000
NPG-520L/NPG-R520L/NPG-G520L/SPG-520L 5 – 20% 7,000 – 400,000 50 – 1,000
NPG-1020L/NPG-R1020L 10 – 20% 7,000 – 200,000 20 – 500
PAGEL分画分子量範囲
Sample Wells
Sample Loading Volume		 12 wells (Width 4.5 mm)
Maximum Loading Volume: about 28μL/well		 18 wells (Width 2.9 mm)
Maximum Loading Volume: about 15μL/well
Compatible with Standard Multi-channel Pipette		 21 wells (Width 1.8 mm)
Maximum Loading Volume: about 10μL/well
Transportation/ Preservation Condition Can be transported in room temperature. Should be stored in a refrigerator (Caution: NOT to be frozen)
Shelf Life 6 months when stored in a refrigerator (NOT to be frozen)
Package 10 plates per box

Note 1: Gels do NOT contain SDS, except for SPG-520L. Electrophoresis with buffer solution containing 24mM Tris + 192mM Glycine + 0.1% SDS is Laemmli method, and one without SDS is Ornstein-Davis method.
Note 2: Use buffer solution containing 25mM Tris and 192mM Glycine for DNA electrophoresis.
Note 3: R-type PAGEL (18 wells) and G-type PAGEL (21 wells) are order-made and the orders are received in every 5 boxes.
Note 4: PAGEL give slightly different electrophoretic patterns to e-PAGEL due to their manufacturing process. e-PAGEL is more cost focused, however it does NOT mean downgrade of quality.

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