WSE-7421 EzSubcell Extract

WSE-7421 EzSubcell Extract kit separates and extracts Cytosol, Nuclei, Membrane, insoluble protein from mammalian cells.

Subcellular extraction kit
WSE-7421 EzSubcell Extract

Overview of the subcellular extraction procedure.

Overview of the subcellular extraction procedure.

Purpose and Application
  • Electrophoresis/ Western blotting/ ImmunoprecipitationELISA/ chromatin immunoprecipitation assay/ Enzyme activity/ etc.
  • Sample preparation for electrophoresis, immunoprecipitation, ELISA, chromatin immunoprecipitation assay, enzyme activity experiments, etc.
  • Simple operation by centrifuging after addition of four extraction buffers stepwisely.
  • Additionally required material is only cooled centrifuge( to10,000 x g).
  • Process for all fractionation is completed in 3 hours. Both Fractionated cell from mammalian tissue and frozen cell are available.
Product Number WSE-7421
Product Name EzSubcell Extract
Compornents ・Extraction buffer; HEPES, detergent
・Protease inhibitor (100×):Aprotinin, PepstatinA, Leupeptin
・DNase I
Applicable amounts 50 samples, up to 2.0×10 7 cells/sample
Storage periode 1 year
Package ・Extraction buffer 1:50mL
・Extraction buffer 2:50mL
・Extraction buffer 3:25mL
・Extraction buffer 4:25mL
・Protease inhibitor (100×):1.25mL
・DNase I:0.25mL

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